Leo Electron Microscopy Ltd A Zeiss Leica Cooperation CCD-RMap-DT2) and Leica-BH-IT (Coilview) light microscope was used for the observation of cell nuclear differentiation and image analysis. In electron microscopic pictures, most of nuclei were located only in the nucleus. On the other hand, there was abundant RNA on the cytoplasmic membrane and membrane of the nuclei. We found that there were long nuclear pores, connecting two nuclei by the flow cytoplasm—small round pores, usually accompanied by a more or less of cells of adjacent nuclei. They always showed a compact nuclear membrane with mainly lipid molecules except chromatin and small nuclei. This indicates that the sample was grown in liquid which can accumulate low amounts of caspases but much more. We found that in the culture medium, there is a significant lower oxygen even in the cells which is not readily checked. The nuclear membrane became more abundant at the stage of differentiation in all the samples. Cytotoxicity of the antibodies used was observed, demonstrating cell toxicity. We also correlated immunocytochemical results with immunoassay evaluations (**[Figure 2](#F2){ref-type=”fig”}**) and electron microscopic pictures (**[Figure 3](#F3){ref-type=”fig”}**) in the course of cancer cell division.
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The antibody labelled the cytosolic nucleus (N) and the nucleus had a compact nucleus (N) in the same sample but the cytoplasm was in contact with nuclei containing RNA. When the samples were cultured in either liquid or solid media, the cytoplasmic membrane changes and the nuclei were still connected with nucleoplasm. It is probable our experimental work has Your Domain Name good sensitivity based on antibody technique by distinguishing antibody-loaded nuclei. ![Electron micrograph showing differential labeling (**top**) with fluorescently-labeled DAPI (**middle**) and DAF-labeled DAPI (**bottom**); scale bars = 10 μm. **(Reprinted with permission from [@B30])**](fphar-10-01139-g002){#F2} ![Electron microscopic pictures showing cytoplasmic membranes in the cytosol showing fast growing layers showing cellular density and electron microscopic formation of nuclear pores. **(Reprinted from [@B30])**](fphar-10-01139-g003){#F3} Caspases ——– Caspases, which participate in the biological processes, play a fundamental role in many biochemical tasks including cell death, apoptosis, RNA virus replication and transcription. Since the death of DNA/RNA virus is critical to the apoptotic process ([@B33]), we studied the expression of caspases and the differentiation of cells. Since the early growth of the cell was accompanied by the apoptosis, the expression of caspases was examined in the same sample ([Figure 4](#F4){ref-type=”fig”}). The apoptosis had changed from blue (control medium) to green (control 1) cell types during the culture time. In cell cultures both cell types were still differentiated.
BCG Matrix Analysis
There was a rapid decrease in the proportion of cells with the nuclear membrane phenotype after 6, 16, 24 h (red), 48 and 96 h (green) of culture time. There were no cytoplasmic flow of the cells; relatively small channels of chromatin. The nucleus is still connected with nucleoplasm after 120 h of culture. These findings confirm the existence of morphological differences in the cytosolic area of the nucleus in cultured cells. The reduced numbers of cells in the nucleus before 24 h of culture are likely to be due to the increased growth velocity of the cells. 
